Gallo obtained his B.S. in Biochemistry and Biophysics at the University of Pittsburgh and his M.S. in Biochemistry from the University of Pittsburgh School of Medicine. He received his Ph.D. at Cornell University in microbiology where he studied the directed evolution of metabolic pathways. His post-doctoral research at the University of Wisconsin-Madison School of Pharmacy focused on the investigation of the elucidation of the genetic pathway for the synthesis of daunorubicin, a potent anti-cancer agent.
Recent research efforts focus on environmental microbiology where he has two major areas of interest. He has been analyzing antibiotic resistance genes in the environment, namely from Staphylococci found associated with white tail deer. Another field of research involves biofilm development on plastic in aquatic environments. Dr. Gallo is also active in science education, especially in the sphere of intrinsic motivation.